Study of carcinogenic activity of carnosic acid and rosmarinic acid in cancer cell line of Hep- G2
In this experimental study, Hep -G2 cells were cultured in DMEM supplemented containing bovine fetal serum and antibiotics. Cells with double dilution were then cultured from 0 to 00 μM for 22 h and viability of cells was determined by MTT method. In order to evaluate activity of caspase3 and 9 enzymes after 22 hours of incubation of the cells with treated materials, cells were centrifuged and cell lysis solution was added. This mixture was centrifuged and then an appropriate substrate was added into each enzyme and after incubation, absorbance of the resulted solution was read at 204 nm using a spectrophotometer device. To measure the level of ceramide, a recombinant acid ceramidase enzyme and naphthalene-2, 3-dialdehyde, which is fluorescent and is connected to sphingosine resulted…
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