Expression of recombinant human Interleukin 2 (rhIL-2) in E. coli
- takbook
- مهندسی مواد
- 14th سپتامبر 2016
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Expression of recombinant human Interleukin 2 (rhIL-2) in E. coli
Background: interleukin-2 (IL-2) is a protein consisted of 132 amino acids (MW = 1.53 kDa). Aldesleukin is the synthetic form of the protein which is used as an effective function for immuno system. Current study was aimed to investigate the expression of rhIL-2 in E. coli BL21 (DE3) expression system in order to produce an active recombinant form of the protein. Methods: Firstly codon optimization was done for hIL-2 gene. Then the gene was synthesized and inserted in pET-22a vector by a cutting strategy included NdeI and BamHI-HF enzymes. In the next step, gene was run in agarose gel and purified. The constructed expression cassette was transformed into E. coli BL21(DE3) cells through CaCl2 heat shock method. Identification and confirmation of the transformed colonies were performed…
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