Evaluation of MT1XT20 Single Quasi-Monomorphic Mononucleotide Marker for characterizing Microsatellite Instability in Persian Lynch Syndrome patients
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- پزشکی عمومی
- 16th سپتامبر 2016
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Evaluation of MT1XT20 Single Quasi-Monomorphic Mononucleotide Marker for characterizing Microsatellite Instability in Persian Lynch Syndrome patients
Background Colorectal malignancies with high microsatellite instability (MSI-H), either hereditary (Lynchsyndrome) or sporadic, demonstrate better prognosis, altered response to 5FU chemotherapy and altered operativeapproach. It is now recommended to perform MSI testing for all new cases of colorectal cancer regardless of beingcategorized as hereditary or sporadic. For MSI detection, immunohistochemistry or PCR-based protocols using acohort of various sets of STR markers are recommended. Here we aimed to evaluate a simplified protocol using justa single STR marker, MT1XT20 mononucleotide repeat, for detection of MSI in Lynch syndrome patients. Promegafive- marker MSI testing panel and Immunohistochemistry used as gold standard in conjunction of MT1XT20.Methods Colorectal patients with…
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