Tracing of New Delhi Metallo-Beta-Lactamase-1 (NDM-1)-producing Klebsiella pneumoniae among carbapenem resistant clinical isolates at a university hos
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- پزشکی عمومی
- 14th سپتامبر 2016
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Tracing of New Delhi Metallo-Beta-Lactamase-1 (NDM-1)-producing Klebsiella pneumoniae among carbapenem resistant clinical isolates at a university hos
Of 112 isolates obtained from various clinical samples, 49 were selected for carbapenemase detection based on their reduced susceptibility to imipenem or meropenem according to the disc diffusion method. These isolates were screened for carbapenemase and MBL production using the Modified Hodge Test (MHT) and Epsilometer test (E-test) MBL strips. Polymerase chain reaction (PCR) was performed on all 49 isolates using specific primers to detect genes encoding IMP (active on imipenem), VIM (Verona integron-encoded metallo-β-lactamase), SPM-1 (Sao Paulo metallo-β-lactamase) and NDM-1….
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